![]() Assays also have different specificities buffer components, such as detergents, can interfere with the assay. ![]() The sensitivity of the assay will determine the lowest level of protein that can be detected. Generally a linear response over a broad range is desired. The range of the assay should allow you to accurately quantify all protein samples. A protein quantification assay should be easy to use and not be cost prohibitive. Choice of the best method relies on various factors. There are several different methods and kits available for employing a spectrophotometer to determine protein concentration. They are generally easy to ease, sensitive and do not rely on the use of hazardous agents. Spectrophotometric methods for measuring protein concentration are popular assays. While these can be sensitive and accurate assays, the more commonly used assays rely on spectrophotometric determination of protein concentration and these will be the focus of this guide. measurement of nitrogen content or radioactively labeling cells) do not rely upon absorptive properties of the protein sample. There are several different methods for quantifying protein concentration in samples. For accurate determine of relative amounts of protein expression, the same amount of protein must be loaded into each lane. Proper quantitation is also critical when performing semi-quantitative or quantitative Westerns. This avoids overloading the lane but still allows adequate detection of the protein of interest. Accurate quantitation of the sample will allow you to load the proper amount of protein in each lane. ![]() After lysis of cells, it is important to determine the total protein concentration of the sample. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |